Human Histocompatibility 2-K1-K region, H2-K1 ELISA Kit from MyBioSource.com

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Human Histocompatibility 2-K1-K region, H2-K1 ELISA Kit

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Description

Intended Uses: This sandwich kit is for the accurate quantitative detection of human Histocompatibility 2-K1-K Region (also known as H2-K1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human H2-K1 antibody. H2-K1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human H2-K1 Antibody is added and binds to H2-K1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated H2-K1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human H2-K1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm